Two New Flex-Seq-EX-L High Throughput Genotyping Platforms for Blueberry and Cranberry
One of VacCAP’s objectives is to leverage newly developed pangenomes of both blueberry and cranberry to design high-throughput genotyping platforms for these two crops that are optimized for high resolution genetic studies. The pangenome consists of core genes, those shared by all cultivars, and auxiliary genes, genes unique to only a subset of cultivars. After assessing current technologies, the Vaccinium research and breeding community selected a targeted genotyping platform, Flex-Seq EX-L, for cost effectiveness, flexibility, ability to capture single nucleotide polymorphisms (SNPs), and generate genotype and haplotype data in the tetraploid blueberry and diploid cranberry. We will present the development and evaluation of these Flex Seq EX-L (22K in blueberry and 17K in cranberry) in their respective 192-member diversity set. These two high throughput platforms are valuable resources for genome-wide association and other genetic studies for the blueberry and cranberry communities.
Presenters: Dr. Nahla Bassil and Dr. Shaun Clare, USDA-ARS, NCGR
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