Monitoring for Spotted Wing Drosophila Using Salt Water Float Tests
The standard Pacific Northwest approach to SWD control consists of three steps:
Assess individual field risks and factors to get a baseline understanding of what you can expect. Evaluate forecasts, as weather conditions significantly influence SWD egg-laying and breeding. Use trapping to establish long-term comparative risk trends.
Regular fruit sampling for larval extraction tests beginning as fruit begins to color. Focusing on high-risk areas in the field and priority fruits on the plant. Monitor at least twice a week, as increased frequency enhances early larvae detection. Sample fruit from the most susceptible field areas.
Use the information gathered from assessments and monitoring to chose the appropriate tool from the SWD management toolbox.
Numerous factors influence SWD risk at the field level, These ‘risk factors’ play an extremely important role in likelihood of SWD damage in a field. Assessing the field risk is important to understanding how little/much control might be necessary. Risk factors hinge on the closeness of fields to SWD population reservoirs and the field environment’s favorability for SWD movement. These factors can be categorized into landscape, crop, and cultural practices.
Larvae Extraction Procedures Information
Fruit collected should be from the highest risk areas of the field (field edges near border habitats) and from most susceptible location within the plant (the shaded interior of bush where SWD are protected from the elements).
The number of fruit depends on the crop (given different sizes of fruit) and crop stage (how much ripe fruit is easily accessible). For blueberries, the number of fruit should ideally be between 150-300 fruit per samples, strawberries is 30-50, raspberries 60-100, blackberries 40-60. Fruit should be quick to collect and a sample shouldn’t take more than 5-10 minutes to collect. If it takes more than that, there probably isn’t enough ripe fruit to take the sample and/or take a smaller sample size of fruit.
A minimum of 3 samples should be collected from different high risk areas of the field.
Sampling should be done at least 2 times per week in order to catch very early, treatable, and non-economic SWD presence.
Lightly squeeze fruit in the bag just enough to break skins but you don’t want to smoosh the fruit to a pulp because that makes it very hard to analyze.
Optional: Leave fruit in bag in hot sun to heat to do initial larvae inspection. Heating will cause larvae, if present, to emerge from fruit and will be actively moving and easy to spot.
Solution is: 1 cup of salt per gallon of water
Alternative solution is using sugar at 2.5c per 1 gal of water. Advantage of sugar is a longer survival time of larvae in the solution.
For a quick check in the field after a designated period of time (at least 15 minutes)
A final check should be done after allowing sample to soak for 1 hour or more. Use a shallow flat tray to pour bag contents into. Having a large surface area makes it easier to examine for any SWD larvae. Wire mesh can also be used to further separate fruit from solution.
One of the basic tenets of IPM is having a toolbox of management options and utilizing the least intensive option that can address the immediate field need. For many years, the SWD management toolbox had few options outside of chemical controls, but that toolbox has and continues to expand rapidly, greatly reducing the need to rely solely on chemical controls. Here is information on some of these different management options:
Trapping is a helpful tool to track adult populations from year to year and over the course of the season. However, they are inadequate for determining field damage or risk. If trapping keep the following in mind:
Spotted Wing Drosophila (SWD) is a major pest affecting small fruit crops in the Pacific Northwest.
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